Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.

نویسندگان

  • P Schmieder
  • J H Ippel
  • H van den Elst
  • G A van der Marel
  • J H van Boom
  • C Altona
  • H Kessler
چکیده

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.

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عنوان ژورنال:
  • Nucleic acids research

دوره 20 18  شماره 

صفحات  -

تاریخ انتشار 1992